Number One (also known as Vaughan's 'First Wife') was a 1962/63 Fender Stratocaster used by Vaughan for most of his career; it was "rebuilt more times than a custom Chevy." Vaughan always claimed it was a 1959 model, since that date was written on the back of the pick-ups; Rene Martinez, who maintained the guitar since 1980, saw the year 1963 stamped in the body and 1962 on the neck. After purchasing the instrument in 1973 from Ray Henning's Heart of Texas music shop in Austin, Texas, it was his main performing instrument and companion. Vaughan used the guitar on all five of his studio albums and on Family Style.

"Number One" had a neck relief of .012" at the 7th and 9th frets, and leveled out through the remainder of the fingerboard. The fingerboard radius when new would have been 7.25 inches as were all pre CBS curved fingerboard Fenders but SRV's guitar ended up after many refrets and sanding of the fingerboard as 10" and used Dunlop 6100 fretwire. String height was measured to be 5/64" on the high E string and 7/64" on the low E string. Each string had 3 full winds for the best angle at the bone nut.

Yellow was a 1959 Stratocaster formerly owned by Vanilla Fudge's lead guitarist, Vince Martell, who sold it to Charley Wirz. The body was hollowed out to make room for "a shitload of humbuckers," but Wirz fashioned a new pickguard in which he placed a single Fender Strat pickup in the neck position and painted the body yellow. Wirz gave the guitar to Vaughan in 1983 or 1984; it is the guitar with the letters "SRV" on the pickguard under the string.

This was supposedly the guitar played on the album versions of "Honey Bee" and "Tell Me". Yellow was stolen in 1985 at the Albany International Airport in New York, but was later recovered and is now on display in the Las Vegas Hard Rock Cafe.

In late 1983, Vaughan purchased a 1962 sunburst Fender Stratocaster from Charley's Guitar Shop, though he had it repainted by Fender in fiesta red as a custom color option, and simply named the guitar "Red." The guitar remained stock until 1986, when a left-handed neck was installed and "SRV" stickers were applied to the pickguard. In 1989, the neck on "Number One" was unable to withstand more re-fret jobs, replacing it with the original neck from "Red." The next year, following a concert at the Garden State Arts Center in Holmdel, New Jersey, a stanchion fell onto Vaughan's rack of guitars, splitting the neck from "Red" that was installed on "Number One." The neck was replaced the next night.

Main (also known as Hamiltone or the "Couldn't Stand the Weather" guitar) was a custom Fender Stratocaster-style guitar made for Vaughan by James Hamilton in Buffalo, NY. It was presented to Vaughan by James as a gift from Billy Gibbons on April 29, 1984.

This guitar features a two-piece maple body and a "neck-through body" design. It also originally had EMG preamped pickups, but Vaughan didn't like the pickups in it. His next music video was about to be made, which was "Couldn't Stand the Weather", and didn't want to get Number One wet during filming, so he used the Hamiltone for filming. The EMG pickups and Gibson style amber top hat knobs were changed in June 1984.

Its fingerboard is ebony with a mother-of-pearl inlay that read "Stevie Ray Vaughan". The guitar was originally set to be made for Stevie in 1979, but the plan was dropped when Vaughan started using his middle name "Ray", as he was known as "Stevie Vaughan" at the time.

Scotch is a 1961 Fender Stratocaster used by Vaughan for the last 5 years of his life. He acquired this guitar in the fall of 1985, and it is said to have been bought in either Baltimore or "The Boathouse" in Norfolk, Virginia. It was to be a prize at one of Stevie's shows, but he bought the guitar instead and gave away another one of his guitars.

This guitar has a butterscotch colored finish with a non-original tiger-striped pickguard made by Rene Martinez, Vaughan's guitar tech. The tiger-striped pickguard resembled the same pickguard in which Buddy Guy had on his butter-colored guitar at the time.

"Scotch" was stock except for the tiger-striped pickguard where he added his famous "SRV" prismatic stickers.

Stevie used this guitar in place of Number One towards the end of 1990, because of Number One's neck problems.

"Red", one of his other guitars, originally had a right-handed neck, but switched over to a left-handed neck in 1986. Around 1990, because "Number One" was having neck troubles, he took the original neck from "Scotch" and put it on "Number One" and took the original neck from "Red" and put it on "Scotch", leaving the left-handed neck on "Red".

Stevie usually used this guitar on "Leave My Girl Alone", and sometimes "Superstition".

Charley was a white custom-made "Stratocaster-style" guitar built by Charley Wirz, a friend of Vaughan's and owner of Charley's Guitar Shop in Dallas. Wirz built it in late 1983, and placed a neck plate on it engraved "To Stevie Ray Vaughan, more in '84". It had three Danelectro lipstick pickups. Stevie later added the famed topless hula-woman stick on the back of the guitar.  This guitar often was used during "Life Without You", which was itself said to be written as a tribute to Charley Wirz.

Lenny is a 1963 or 1964 Stratocaster, bought for Vaughan by his wife, Lenora, because he didn't have the money to buy it. Originally 3-tone sunburst with a rosewood neck, it was later stripped down to a dark natural finish and re-fitted with a mid-'50s-style maple neck reportedly given to him by Billy Gibbons. Behind the bridge, on the lower bout of the guitar body is a unique inlay, thought to be originally from an early 1900s mandolin. The Fender Custom Shop has produced a limited-edition run of Lenny replicas since December 12, 2007, and they are sold by the Guitar Center for $17,000. The guitar is mainly used for "Lenny". In 2004, Lenny was put up for auction and was sold to Guitar Center for $623,500